It is a technique that separates macromolecules (polymers) based on their hydrodynamic size in solution. The relative molecular weight (molar mass) of an unknown polymer can be determined by calibrating the GPC column set by injecting a series of narrow polymer standards, and subsequently comparing elution volumes. If the GPC system uses advanced detectors such as a light-scattering detector, calibration of the detector itself will provide the absolute measurement of molecular weight.
When monitoring the degradation of biopolymers, GPC/SEC data is critical. However, characterization of biopolymers by GPC/SEC is challenging due to the nature of these materials. Typically such polymers carry various active sites with a range of polarities on their molecular chains, causing difficulties in obtaining complete recovery through common GPC/SEC columns. PolyAnalytik has successfully completed many projects developing multi-detection GPC/SEC methods using proprietary polar and non-polar columns. These columns have provided a tremendous edge for complete characterization of popular biopolymers such as Polylactic Acid (PLA), Polyhydroxybutyrate (PHB), Polycaprolactone (PCL), Chitosan, Beta Glucans, Starches and Cellulosic Materials.